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Response of cell-wall composition and RNA-seq transcriptome to methyl-jasmonate in Brachypodium distachyon callus

Planta, 2018, Vol.248(5), pp.1213-1229 [Peer Reviewed Journal]

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  • Title:
    Response of cell-wall composition and RNA-seq transcriptome to methyl-jasmonate in Brachypodium distachyon callus
  • Author: Hyde, Lucy ; Pellny, Till ; Freeman, Jackie ; Michaelson, Louise ; Simister, Rachael ; McQueen-Mason, Simon ; Mitchell, Rowan
  • Found In: Planta, 2018, Vol.248(5), pp.1213-1229 [Peer Reviewed Journal]
  • Subjects: Arabinoxylan ; Coumaroylation ; Hemicellulose ; Hydroxycinnamic acids ; Jasmonic acid ; Lignin
  • Language: English
  • Description: To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1007/s00425-018-2968-9 Byline: Lucy S. Hyde (1), Till K. Pellny (1), Jackie Freeman (1), Louise V. Michaelson (1), Rachael Simister (2), Simon J. McQueen-Mason (2), Rowan A. C. Mitchell (1) Keywords: Arabinoxylan; Coumaroylation; Hemicellulose; Hydroxycinnamic acids; Jasmonic acid; Lignin Abstract: Main conclusion Methyl-jasmonate induces large increases in p-coumarate linked to arabinoxylan in Brachypodium and in abundance of GT61 and BAHD family transcripts consistent with a role in synthesis of this linkage. Jasmonic acid (JA) signalling is required for many stress responses in plants, inducing large changes in the transcriptome, including up-regulation of transcripts associated with lignification. However, less is known about the response to JA of grass cell walls and the monocot-specific features of arabinoxylan (AX) synthesis and acylation by ferulic acid (FA) and para-coumaric acid (pCA). Here, we show that methyl-jasmonate (MeJA) induces moderate increases in FA monomer,>50% increases in FA dimers, and five--sixfold increases in pCA ester-linked to cell walls in Brachypodium callus. Direct measurement of arabinose acylated by pCA (Araf-pCA) indicated that most or all the increase in cell-wall pCA was due to pCA ester-linked to AX. Analysis of the RNA-seq transcriptome of the callus response showed that these cell-wall changes were accompanied by up-regulation of members of the GT61 and BAHD gene families implicated in AX decoration and acylation two BAHD paralogues were among the most up-regulated cell-wall genes (seven and fivefold) after 24 h exposure to MeJA. Similar responses to JA of orthologous BAHD and GT61 transcripts are present in the RiceXPro public expression data set for rice seedlings, showing that they are not specific to Brachypodium or to callus. The large response of AX-pCA to MeJA may, therefore, indicate an important role for this linkage in response of primary cell walls of grasses to JA signalling. Author Affiliation: (1) 0000 0001 2227 9389, grid.418374.d, Plant Sciences Department, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK (2) 0000 0004 1936 9668, grid.5685.e, Centre for Novel Agricultural Products (CNAP), Department of Biology, University of York, York, YO10 5DD, UK Article History: Registration Date: 02/08/2018 Received Date: 08/05/2018 Accepted Date: 26/07/2018 Online Date: 09/08/2018 Article note: Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-018-2968-9) contains supplementary material, which is available to authorized users.
  • Identifier: ISSN: 0032-0935 ; E-ISSN: 1432-2048 ; DOI: 10.1007/s00425-018-2968-9

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