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Cytological characterization of Jatropha curcas callus in different periods of cultivation

Crop Breeding and Applied Biotechnology, 01 December 2013, Vol.13(4), pp.228-233 [Peer Reviewed Journal]

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  • Title:
    Cytological characterization of Jatropha curcas callus in different periods of cultivation
  • Author: Santos, Dalilhia Nazaré Dos ; Nunes, Claudinéia Ferreira ; Soares, Joyce Dória Rodrigues ; Valente, Thaís Cainã Teixeira ; Alves, Eduardo ; Labory, Cláudia Regina Gontijo ; Pasqual, Moacir
  • Found In: Crop Breeding and Applied Biotechnology, 01 December 2013, Vol.13(4), pp.228-233 [Peer Reviewed Journal]
  • Subjects: Agronomy ; Biotechnology & Applied Microbiology ; In Vitro ; Cinetina E Ultraestrutura ; In Vitro ; Kinetics and Ultrastructure
  • Language: Portuguese
  • Description: The aimed was characterization and determining the time for Jatropha curcas callus subculture. The pattern of J. curcas callus development is sigmoidal with 5 distinct phases, and transplantation should be performed at the end of the deceleration phase (approximately day 28). During callus development, it was observed that at the onset of growth in the exponential phase the cells were internally disorganized; in the linear phase, respiratory metabolism was resumed through reorganization of the first mitochondria; and by the mid-deceleration phase, the cells were entirely active, and several organelles were detected. This organizational status was maintained throughout the stationary phase during which somatic pre-embryos were identified. At the end of the stationary phase, intracellular disruption began, and the cells entered senescence, which characterized the decline phase for the J. curcas calli growth curve.
  • Identifier: ISSN: 1984-7033 ; DOI: 10.1590/S1984-70332013000400002

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