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Callus induction and plant regeneration of Ulex europaeus

Electronic Journal of Biotechnology, 01 July 2012, Vol.15(4), pp.7-7 [Peer Reviewed Journal]

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  • Title:
    Callus induction and plant regeneration of Ulex europaeus
  • Author: Ramírez, Ingrid ; Dorta, Fernando ; Cuadros-Inostroza, Álvaro ; Peña-Cortés, Hugo
  • Found In: Electronic Journal of Biotechnology, 01 July 2012, Vol.15(4), pp.7-7 [Peer Reviewed Journal]
  • Subjects: Biotechnology & Applied Microbiology ; Antioxidants ; Callus Induction ; Fabaceae ; Gorse ; Plant Regeneration ; Ulex Europaeus ; Engineering
  • Language: Portuguese
  • Description: A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite.
  • Identifier: ISSN: 0717-3458 ; E-ISSN: 0717-3458

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