skip to main content
Show Results with:

Sperm associated antigen 9 (SPAG9) a promising therapeutic target of ovarian carcinoma

Tumor Biology, May 2018, Vol.40(5) [Peer Reviewed Journal]

Full text available

  • Title:
    Sperm associated antigen 9 (SPAG9) a promising therapeutic target of ovarian carcinoma
  • Author: Jagadish, Nirmala ; Fatima, Rukhsar ; Sharma, Aditi ; Devi, Sonika ; Suri, Vitusha ; Kumar, Vikash ; Suri, Anil
  • Found In: Tumor Biology, May 2018, Vol.40(5) [Peer Reviewed Journal]
  • Subjects: Spag9 ; CT Antigen ; Therapeutic Target ; Cell Cycle Arrest ; and Apoptosis ; Medicine ; Chemistry
  • Language: English
  • Description: SPAG9 is a novel tumor associated antigen, expressed in variety of malignancies. However, its role in ovarian cancer remains unexplored. SPAG9 expression was validated in ovarian cancer cells by real time PCR and Western blot. SPAG9 involvement in cell cycle, DNA damage, apoptosis, paclitaxel sensitivity and epithelial- mesenchymal transition (EMT) was investigated employing RNA interference approach. Combinatorial effect of SPAG9 ablation and paclitaxel treatment was evaluated in in vitro. Quantitative PCR and Western blot analysis revealed SPAG9 expression in A10, SKOV-3 and Caov3 compared to normal ovarian epithelial cells. SPAG9 ablation resulted in reduced cellular proliferation, colony forming ability and enhanced cytotoxicity of chemotherapeutic agent paclitaxel. Effect of ablation of SPAG9 on cell cycle revealed S phase arrest and showed decreased expression of CDK1, CDK2, CDK4, CDK6, cyclin B1, cyclin D1, cyclin E and increased expression of tumor suppressor p21. Ablation...
  • Identifier: E-ISSN: 1423-0380 ; DOI: 10.1177/1010428318773652

Searching Remote Databases, Please Wait