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Temporal and spatial expression patterns for the tumor suppressor Maspin and its binding partner interferon regulatory factor 6 during breast development.

Development, growth & differentiation, June 2009, Vol.51(5), pp.473-481 [Peer Reviewed Journal]

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  • Title:
    Temporal and spatial expression patterns for the tumor suppressor Maspin and its binding partner interferon regulatory factor 6 during breast development.
  • Author: Bailey, Caleb M ; Margaryan, Naira V ; Abbott, Daniel E ; Schutte, Brian C ; Yang, Baoli ; Khalkhali-Ellis, Zhila ; Hendrix, Mary J C
  • Contributor: Bailey, Caleb M (correspondence author) ; Bailey, Caleb M (record owner)
  • Found In: Development, growth & differentiation, June 2009, Vol.51(5), pp.473-481 [Peer Reviewed Journal]
  • Subjects: Animals–Metabolism ; Blotting, Western–Metabolism ; Cell Culture Techniques–Metabolism ; Cell Line–Metabolism ; Epithelial Cells–Metabolism ; Female–Metabolism ; Humans–Metabolism ; Immunohistochemistry–Metabolism ; Interferon Regulatory Factors–Metabolism ; Mammary Glands, Human–Metabolism ; Mice–Metabolism ; Serpins–Metabolism ; Irf6 Protein, Human ; Irf6 Protein, Mouse ; Interferon Regulatory Factors ; Serpin-B5 ; Serpins
  • Language: English
  • Description: To authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1440-169X.2009.01110.x Keywords: breast cancer; cell differentiation; interferon regulatory factor 6; mammary gland; Maspin Abstract: Interferon regulatory factor 6 (IRF6) is a non-canonical member of the interferon regulatory factor family of transcription factors. We recently identified IRF6 as a novel Maspin-interacting protein in mammary epithelial cells. Maspin is a tumor suppressor in the breast and has also been implicated in mammary gland morphogenesis. To explore a possible role for IRF6 in conjunction with Maspin during mammary gland growth and differentiation, we examined the expression of IRF6 and Maspin during post-utero mammary gland development using a combination of in vitro and in vivo approaches. The data revealed that the expression of IRF6 and Maspin is temporally and spatially regulated throughout mammary gland development, with maximal expression of both proteins occurring in fully differentiated, lactating lobuloalveolar cells. We further show that IRF6 adopts a lumenal localization pattern following complete epithelial cell polarization and present new evidence for the secretion of IRF6 into the milk. These results support the hypothesis that IRF6 and Maspin are important for mammary epithelial cell differentiation, and advance our understanding of the Maspin-IRF6 partnership during normal mammary gland development. Author Affiliation: (1)Children's Memorial Research Center, (2)Cancer Biology and Epigenomics Program, Robert H. Lurie Comprehensive (3)Cancer Center and the (4)Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60614-3394, and Departments of (5)Pediatrics and (6)Obstetrics and Gynecology, Roy A. and Lucille J. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA Article History: Received 19 September 2008; revised 13 March 2009; accepted 16 March 2009. Article note: (*) Author to whom all correspondence should be addressed., Email: mjchendrix@childrensmemorial.org, zellis@childrensmemorial.org
  • Identifier: E-ISSN: 1440-169X ; DOI: 10.1111/j.1440-169X.2009.01110.x

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