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A poly(A)-specific ribonuclease directly regulates the poly(A) status of mitochondrial mRNA in Arabidopsis

Nature Communications, Aug 2013, Vol.4, p.2247 [Peer Reviewed Journal]

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  • Title:
    A poly(A)-specific ribonuclease directly regulates the poly(A) status of mitochondrial mRNA in Arabidopsis
  • Author: Hirayama, Takashi ; Matsuura, Takakazu ; Ushiyama, Sho ; Narusaka, Mari ; Kurihara, Yukio ; Yasuda, Michiko ; Ohtani, Misato ; Seki, Motoaki ; Demura, Taku ; Nakashita, Hideo ; Narusaka, Yoshihiro ; Hayashi, Shimpei
  • Found In: Nature Communications, Aug 2013, Vol.4, p.2247 [Peer Reviewed Journal]
  • Subjects: Arabidopsis -- Enzymology ; Exoribonucleases -- Metabolism ; Poly A -- Metabolism ; RNA, Messenger -- Metabolism
  • Language: English
  • Description: Coordination of gene expression in the organelles and the nucleus is important for eukaryotic cell function. Transcriptional and post-transcriptional gene regulation in mitochondria remains incompletely understood in most eukaryotes, including plants. Here we show that poly(A)-specific ribonuclease, which influences the poly(A) status of cytoplasmic mRNA in many eukaryotes, directly regulates the poly(A) tract of mitochondrial mRNA in conjunction with a bacterial-type poly(A) polymerase, AGS1, in Arabidopsis. An Arabidopsis poly(A)-specific ribonuclease-deficient mutant, ahg2-1, accumulates polyadenylated mitochondrial mRNA and shows defects in mitochondrial protein complex levels. Mutations of AGS1 suppress the ahg2-1 phenotype. Mitochondrial localizations of AHG2 and AGS1 are required for their functions in the regulation of the poly(A) tract of mitochondrial mRNA. Our findings suggest that AHG2 and AGS1 constitute a regulatory system that controls mitochondrial mRNA poly(A) status in Arabidopsis.
  • Identifier: E-ISSN: 20411723 ; DOI: 10.1038/ncomms3247

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