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Major differences in specificity among naturally occurring human IgG-subclass anti-IgE autoantibodies

The Journal of Allergy and Clinical Immunology, 1995, Vol.95(5), pp.1011-1019 [Peer Reviewed Journal]

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  • Title:
    Major differences in specificity among naturally occurring human IgG-subclass anti-IgE autoantibodies
  • Author: Magnusson, Carl G.M
  • Found In: The Journal of Allergy and Clinical Immunology, 1995, Vol.95(5), pp.1011-1019 [Peer Reviewed Journal]
  • Subjects: Anti-Ige ; Assay ; Atopic ; Autoantibody ; Ige ; Igg4 ; Igg Subclass ; Specificity ; Medicine
  • Language: English
  • Description: The specificity of naturally occurring IgG-subclass anti-IgE autoantibodies (a-IgE Ab) was studied by ELISA inhibition assay with a highly purified IgE-DES myeloma protein as a solid-phase antigen. Because the IgG isotypes differ in effector functions, detailed specificity studies of IgG subclass anti-IgE antibodies might clarify their putative role. Selected sera containing a high concentration of a single IgG a-IgE Ab subclass were allowed to react with purified immunoglobulins of all five classes including five different IgE myeloma proteins and papain-derived Fabϵ and Fcϵ fragments of IgE-DES. The inhibition results indicated that IgG1, IgG2, and IgG3 a-IgE Ab reacted in a low-affinity reaction with IgE myeloma protein–restricted determinants because only IgE-DES was inhibitory. Moreover, the reacting epitopes were heat resistant (2 hours, 56° C) and localized in the Fabϵ-DES fragment. In sharp contrast,...
  • Identifier: ISSN: 0091-6749 ; E-ISSN: 1097-6825 ; DOI: 10.1016/S0091-6749(95)70102-8

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