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In situ 3D characterization of historical coatings and wood using multimodal nonlinear optical microscopy

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  • Title:
    In situ 3D characterization of historical coatings and wood using multimodal nonlinear optical microscopy
  • Author: Gaël Latour ; Jean-Philippe Echard ; Marie Didier ; Marie-Claire Schanne-Klein
  • Subjects: Space Science ; Medicine ; Cell Biology ; Environmental Sciences Not Elsewhere Classified ; Ecology ; Science Policy ; Plant Biology ; Virology
  • Publication Details: Figshare
  • Description: We demonstrate multimodal nonlinear optical imaging of historical artifacts by combining Second Harmonic Generation (SHG) and Two-Photon Excited Fluorescence (2PEF) microscopies. We first identify the nonlinear optical response of materials commonly encountered in coatings of cultural heritage artifacts by analyzing one- and multi-layered model samples. We observe 2PEF signals from cochineal lake and sandarac and show that pigments and varnish films can be discriminated by exploiting their different emission spectral ranges as in luminescence linear spectroscopy. We then demonstrate SHG imaging of a filler, plaster, composed of bassanite particles which exhibit a non centrosymmetric crystal structure. We also show that SHG/2PEF imaging enables the visualization of wood microstructure through typically 60 µm-thick coatings by revealing crystalline cellulose (SHG signal) and lignin (2PEF signal) in the wood cell walls. Finally, in situ multimodal nonlinear imaging is demonstrated in a historical violin. SHG/2PEF imaging thus appears as a promising non-destructive and contactless tool for in situ 3D investigation of historical coatings and more generally for wood characterization and coating analysis at micrometer scale....
  • Identifier: DOI: 10.6084/m9.figshare.c.3764039 ; Related DOI: 10.1364/OE.20.024623
  • Creation Date: 2012

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