skip to main content
Show Results with:

Effects of antioxidants on apoptosis induced by dasatinib and nilotinib in K562 cells

Damiano, Sara et al.

Journal of cellular biochemistry. Volume 119:Issue 6 (2018); pp 4845-4854 -- Wiley Periodicals, Inc

Online access

  • Title:
    Effects of antioxidants on apoptosis induced by dasatinib and nilotinib in K562 cells
  • Author: Damiano, Sara;
    Montagnaro, Serena;
    Puzio, Maria V.;
    Severino, Lorella;
    Pagnini, Ugo;
    Barbarino, Marcella;
    Cesari, Daniele;
    Giordano, Antonio;
    Florio, Salvatore;
    Ciarcia, Roberto
  • Found In: Journal of cellular biochemistry. Volume 119:Issue 6 (2018); pp 4845-4854
  • Journal Title: Journal of cellular biochemistry
  • Subjects: Cytochemistry--Periodicals; antioxidants--apoptosis--dasatinib--nilotinib; Dewey: 572
  • Rights: legaldeposit
  • Publication Details: Wiley Periodicals, Inc
  • Abstract: Abstract:

    In clinical practice for the treatment of chronic myeloid leukemia, second generation of tyrosine kinase inhibitors such as Nilotinib (NIL) specific and potent inhibitor of the BCR/ABL kinase and Dasatinib (DAS) a inhibitor of BCR/ABL and Src family kinase were developed to clinically overcome imatinib resistance. In this study, we wanted to test the ability of some antioxidants such Resveratrol (RES) or a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) or δ‐tocotrienol (δ‐TOCO) to interact with DAS and NIL on viability, reactive oxygen species (ROS) production, lipid peroxidation, and apoptosis. To test the possible mechanisms of action of such antioxidants, we utilized N‐acetyl‐L‐cysteine (NAC) a specific inhibitor ROS production or PP1 a specific Src tyrosine kinase inhibitor or BAPTA a specific chelator of intracellular calcium. Our data demonstrated: 1) RES, rMnSOD, δ‐TOCO, and NAC, at dose used, significantly reduced the intracellular levels of MDA induced by DAS or NIL; 2) RES, rMnSOD, and δ‐TOCO increased the intracellular ROS levels; 3) The increase ROS levels is related to higher levels of oligonucleosomesi induced by DAS and NIL and that NAC significantly reduced this activity. Interestingly, our data showed that apoptotic activity of DAS and NIL have significantly increased the production of oligonucleosomes by triggering excessive ROS generation as well as functionality of SERCA receptors.

    Abstract :

    Interestingly, our data showed that apoptotic activity of DAS and NIL have significantly increased the production of oligonucleosomes by triggering excessive ROS generation as well as functionality of SERCA receptors.


  • Identifier: System Number: LDEAvdc_100062039247.0x000001; Journal ISSN: 0730-2312; 10.1002/jcb.26686
  • Publication Date: 2018
  • Physical Description: Electronic
  • Shelfmark(s): ELD Digital store

Searching Remote Databases, Please Wait