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Upregulated glutathione transferase omega-1 correlates with progression of urinary bladder carcinoma

Djukic, Tatjana et al.

Redox report. Volume 22:Number 6 (2017); pp 486-492 -- Maney Publishing on behalf of the Society for Free Radical Research (Australasia)

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  • Title:
    Upregulated glutathione transferase omega-1 correlates with progression of urinary bladder carcinoma
  • Author: Djukic, Tatjana;
    Simic, Tatjana;
    Pljesa-Ercegovac, Marija;
    Matic, Marija;
    Suvakov, Sonja;
    Coric, Vesna;
    Dragicevic, Dejan;
    Savic-Radojevic, Ana
  • Found In: Redox report. Volume 22:Number 6 (2017); pp 486-492
  • Journal Title: Redox report
  • Subjects: Free radicals (Chemistry)--Pathophysiology--Periodicals; Oxidation, Physiological--Periodicals; Glutathione transferase omega 1--glutathione--glutathionylation--transitional cell carcinoma--uroepithelium; Dewey: 541.224
  • Rights: legaldeposit
  • Publication Details: Maney Publishing on behalf of the Society for Free Radical Research (Australasia)
  • Abstract: ABSTRACT:

    Objectives: Newly discovered glutathione transferase omega 1 (GSTO1-1) plays an important role in the glutathionylation cycle, a significant mechanism of protein function regulation. GSTO1-1 expression pattern has not been studied in transitional cell carcinoma (TCC), as yet.

    Methods: A total of 56 TCC tumor and corresponding non-tumor specimens were investigated. Glutathione content and thioltransferase activity were measured spectrophotometrically. Protein-glutathione mixed disulfides were measured fluorimetrically. GSTO1-1 expression was determined by immunoblot and qPCR. Immunoprecipitation with GSTO1-1 antibody was followed by immunoblot using anti-GSTO1, GSTP1, c-Jun, JNK, Akt, phospho-Akt, and ASK1 antibody, while for the total S-glutathionylation levels non-reducing electrophoresis was performed.

    Results: The contents of reduced glutathione and thioltransferase activity were significantly increased in tumor compared to non-tumor tissue. The increased GSTO1 expression in tumor tissue showed clear correlation with grade and stage. However, decreased total protein glutathionylation level in tumor compared to non-tumor samples was found. Immunoprecipitation has shown an association of GSTO1-1 with GSTP1, Akt, phospho-Akt, and ASK1 proteins.

    Conclusions: GSTO1 deglutathionylase activity suggests its potential important role in redox perturbations present in TCC. Increased GSTO1-1 expression might contribute to TCC development and/or progression supporting the notion that GSTO1-1 may be a promising novel cancer target.


  • Identifier: System Number: LDEAvdc_100050331162.0x000001; Journal ISSN: 1351-0002; 10.1080/13510002.2017.1299909
  • Publication Date: 2017
  • Physical Description: Electronic
  • Shelfmark(s): ELD Digital store

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