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Comb-shaped polymer grafted with REDV peptide, PEG and PEI as targeting gene carrier for selective transfection of human endothelial cells1

Wang, Haixia et al.

Journal of materials chemistry: materials for biology and medicine. B. Volume 5:Issue 7 (2017); pp 1408-1422 -- RSC Publishing

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  • Title:
    Comb-shaped polymer grafted with REDV peptide, PEG and PEI as targeting gene carrier for selective transfection of human endothelial cells1
  • Author: Wang, Haixia;
    Li, Qian;
    Yang, Jing;
    Guo, Jintang;
    Ren, Xiangkui;
    Feng, Yakai;
    Zhang, Wencheng
  • Found In: Journal of materials chemistry: materials for biology and medicine. B. Volume 5:Issue 7 (2017); pp 1408-1422
  • Journal Title: Journal of materials chemistry: materials for biology and medicine. B
  • Subjects: Biomedical materials--Research--Periodicals; Chemistry, Analytic--Periodicals; Materials--Periodicals; Dewey: 543.0284
  • Rights: legaldeposit
  • Publication Details: RSC Publishing
  • Abstract: Abstract :

    Several REDV peptide molecules are covalently linked onto an amphiphilic block copolymer to obtain REDV-modified polycationic polymer as a gene carrier with targeting function. The targeting gene complexes show high cell recognition and binding affinity to human endothelial cells.

    Abstract :

    If plasmid complexes prepared from targeting carriers have endothelial cell selectivity and high transfection efficiency, they can specifically enhance rapid endothelialization by delivering the corresponding gene plasmids. A high content of functional groups in the carriers benefits high selectivity efficiency. Herein, we have synthesized a comb-shaped polymer bearing several Arg-Glu-Asp-Val (REDV) peptides and poly(ethylene glycol) as a pEGFP-ZNF580 gene carrier with cell-type recognition of human endothelial cells. An amphiphilic block copolymer of poly(2-hydroxyethyl methacrylate)- block -poly(ε-caprolactone)- graft -poly(ethylene glycol)- graft -poly(ethyleneimine) conjugated with REDV peptide (PHEMA- b -PCL- g -PEG- g -PEI-REDV) is synthesized, and nanoparticles of it are prepared by polymer self-assembly. This polycationic PHEMA- b -PCL- g -PEG- g -PEI-REDV carrier effectively condenses pEGFP-ZNF580 plasmid to form REDV-targeted complexes and protects pEGFP-ZNF580 integrity from enzymatic hydrolysis. These REDV-targeted complexes exhibit low cytotoxicity but high transfection efficiency to EA.hy926 cells compared with non-targeted complexes (PHEMA- b -PCL- g -PEG- g -PEI/pEGFP-ZNF580) as demonstrated by MTT assay, fluorescence-activated cell sorting analysis and fluorescence analysis. Furthermore, the relative protein level of endothelial cells transfected by REDV-targeted complexes is higher than that by non-targeted complexes. Therefore, REDV-bearing carriers may have potential as effective and targeting transfer carriers for pEGFP-ZNF580 plasmid, and their complexes can be used in the endothelialization of artificial vascular grafts.


  • Identifier: System Number: LDEAvdc_100044271716.0x000001; Journal ISSN: 2050-750X; 10.1039/c6tb02379g
  • Publication Date: 2017
  • Physical Description: Electronic
  • Shelfmark(s): ELD Digital store

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