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Pull‐Down of Metalloproteins in Their Native States by Using Desthiobiotin‐Based Probes

Ngo, Chinh

Chembiochem: a European journal of chemical biology. Volume 20:Number 8 (2019, April); pp 1003-1007 -- Wiley-VCH

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  • Title:
    Pull‐Down of Metalloproteins in Their Native States by Using Desthiobiotin‐Based Probes
  • Author: Ngo, Chinh;
    Mehta, Radhika;
    Aggarwal, Kanchan;
    Fikes, Audrey G.;
    Santos, Ines C.;
    Greer, Sylvester M.;
    Que, Emily L.
  • Found In: Chembiochem: a European journal of chemical biology. Volume 20:Number 8 (2019, April); pp 1003-1007
  • Journal Title: Chembiochem: a European journal of chemical biology
  • Subjects: Biochemistry--Periodicals; Molecular biology--Periodicals; Pharmaceutical chemistry--Periodicals; carbonic anhydrase--desthiobiotin--metalloproteins--proteomics--sulfonamide; Dewey: 572
  • Rights: Licensed
  • Publication Details: Wiley-VCH
  • Abstract: Abstract:

    One‐third of all proteins are estimated to require metals for structural stability and/or catalytic activity. Desthiobiotin probes containing metal binding groups can be used to capture metalloproteins with exposed active‐site metals under mild conditions so as to prevent changes in metallation state. The proof‐of‐concept was demonstrated with carbonic anhydrase (CA), an open active site, Zn 2+ ‐containing protein. CA was targeted by using sulfonamide derivatives. Linkers of various lengths and structures were screened to determine the optimal structure for capture of the native protein. The optimized probes could selectively pull down CA from red blood cell lysate and other protein mixtures. Pull‐down of differently metallated CAs was also investigated.

    Avoiding the destruct button: One‐third of all proteins are estimated to require metals for structural stability and/or catalytic activity. Desthiobiotin probes with metal‐binding sulfonamide groups enable the pull‐down of the metalloprotein carbonic anhydrase under mild conditions without destroying the protein metallation state.


  • Identifier: System Number: ETOCvdc_100090613059.0x000001; Journal ISSN: 1439-4227
  • Publication Date: 2019
  • Physical Description: Electronic
  • Shelfmark(s): 3133.490980
  • UIN: ETOCvdc_100090613059.0x000001

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