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CXCL12 and CXCR4, but not CXCR7, are primarily expressed by the stroma in head and neck squamous cell carcinoma

Clatot, Florian et al.

Pathology -- Elsevier B.V. -- Volume: 47 Issue: 1; (pages 45-50) -- 2015

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  • Title:
    CXCL12 and CXCR4, but not CXCR7, are primarily expressed by the stroma in head and neck squamous cell carcinoma
  • Author: Clatot, Florian;
    Cornic, Marie;
    Berghian, Anca;
    Marchand, Vinciane;
    Choussy, Olivier;
    El Ouakif, Faissal;
    François, Arnaud;
    Ruminy, Philippe;
    Laberge-Le-Couteulx, Sophie;
    Picquenot, Jean-Michel;
    Jardin, Fabrice
  • Found In: Pathology. Volume 47:Number 1(2015); 201501; 45-50
  • Journal Title: Pathology
  • Subjects: Pathology; LCSH: Pathology; Dewey: 616.0705
  • Rights: Licensed
  • Publication Details: Elsevier B.V.
  • Abstract: SummaryThe CXCL12/CXCR4 axis is involved in numerous models of metastatic dissemination, including head and neck squamous cell carcinoma (HNSCC). We assessed the relative expressions ofCXCL12,CXCR4andCXCR7in the stroma and the tumour of HNSCC, and evaluated the methylation status of theCXCL12promoter. Snap-frozen, HPV negative HNSCC samples were micro-dissected to isolate the tumoural and stromal compartments. The expression levels ofCXCL12,CXCR4andCXCR7were assessed by qRT-PCR, and the methylation level of theCXCL12promoter was evaluated by pyrosequencing. In total, 23 matched tumour/stroma samples were analysed. Higher expressions ofCXCR4andCXCL12were observed in the stroma (p= 0.012 andp< 0.0001, respectively). No significant difference in expression was observed forCXCR7. A high meth-ylation level (>40%) of theCXCL12promoter was observed in onlya few tumoural samples (5/23) and was associated with a lower expression of the gene (p= 0.03). Stromal cells, rather than the tumour itself, are mainly responsible for the expression of bothCXCL12andCXCR4expression in HNSCC.CXCR7expression did not differ between the two compartments and was not related toCXCL12orCXCR4expression. Finally, the methylation of theCXCL12promoter could only explain the low intra-tumoural expression of this gene in 20% of cases.
  • Identifier: Journal ISSN: 0031-3025
  • Publication Date: 2015
  • Physical Description: Electronic
  • Shelfmark(s): ELD Digital store
    6412.810000
  • UIN: ETOCvdc_100054087180.0x000001

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