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Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats

Guzen, F. P. et al.

Neuroscience letters. VOL 616, ; 2016, 43-48 -- Elsevier Science B.V., Amsterdam. (pages 43-48) -- 2016

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  • Title:
    Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats
  • Author: Guzen, F. P.;
    de Araújo, D. P.;
    de Souza Lucena, E. E.;
    de Morais, H. c.;
    de Paiva Cavalcanti, J. R.;
    do Nascimento, E. S.;
    de Oliveira Costa, M. S.;
    Cavalcante, J. S.
  • Found In: Neuroscience letters. VOL 616, ; 2016, 43-48
  • Journal Title: Neuroscience letters.
  • Subjects: Medicine; Biotechnology; Pharmaceutical Chemistry; LCC: QP351; Dewey: 612.8
  • Publication Details: Elsevier Science B.V., Amsterdam.
  • Language: English
  • Abstract: Highlights•Transected spinal cord as a model for study of DRG regeneration.•Peripheral nerve grafts as a favorable environment to DRG neuroprotection.•FGF-2 potentiates neuroprotective effect in DRG after spinal cord injury.•FGF-2 plus sciatic nerve fragment improve DRG plasticity of rats submitted to complete transections of spinal cord.AbstractNeurotrophic factors and peripheral nerves are known to be good substrates for bridging CNS trauma. The involvement of fibroblast growth factor-2 (FGF-2) activation in the dorsal root ganglion (DRG) was examined following spinal cord injury in the rat. We evaluated whether FGF-2 increases the ability of a sciatic nerve graft to enhance neuronal plasticity, in a gap promoted by complete transection of the spinal cord. The rats were subjected to a 4mm-long gap at low thoracic level and were repaired with saline (Saline or control group,n=10), or fragment of the sciatic nerve (Nerve group,n=10), or fragment of the sciatic nerve to which FGF-2 (Nerve+FGF-2 group,n=10) had been added immediately after lesion. The effects of the FGF-2 and fragment of the sciatic nerve grafts on neuronal plasticity were investigated using choline acetyl transferase (ChAT)-immunoreactivity of neurons in the dorsal root ganglion after 8 weeks. Preservation of the area and diameter of neuronal cell bodies in dorsal root ganglion (DRG) was seen in animals treated with the sciatic nerve, an effect enhanced by the addition of FGF-2. Thus, the addition of exogenous FGF-2 to a sciatic nerve fragment grafted in a gap of the rat spinal cord submitted to complete transection was able to improve neuroprotection in the DRG. The results emphasized that the manipulation of the microenvironment in the wound might amplify the regenerative capacity of peripheral neurons.
  • Identifier: Journal ISSN: 0304-3940
  • Publication Date: 2016
  • Physical Description: Physical
  • Shelfmark(s): 6081.562000
  • UIN: ETOCRN376685984

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