skip to main content
Show Results with:

HLA-DR Binding Prediction and Experimental Evaluation of T-Cell Epitopes of Mycolyl Transferase 85B (Ag85B), a Major Secreted Antigen of Mycobacterium tuberculosis

Mustafa, A. S. et al.

Medical principles and practice. VOL 14; NUMBER 3, ; 2005, 140-146 -- S. Karger AG (pages 140-146) -- 2005

Online access

  • Title:
    HLA-DR Binding Prediction and Experimental Evaluation of T-Cell Epitopes of Mycolyl Transferase 85B (Ag85B), a Major Secreted Antigen of Mycobacterium tuberculosis
  • Author: Mustafa, A. S.;
    Abal, A. T.;
    Shaban, F.;
    El-Shamy, A. M.;
    Amoudy, H. A.
  • Found In: Medical principles and practice. VOL 14; NUMBER 3, ; 2005, 140-146
  • Journal Title: Medical principles and practice.
  • Subjects: Medicine; Biotechnology; Pharmaceutical Chemistry; LCC: R895; Dewey: 610
  • Publication Details: S. Karger AG
  • Language: English
  • Abstract: Abstract Objective: To identify T-cell epitopes of Ag85B by analysis of its sequence for prediction to bind HLA-DR alleles and evaluate the predicted peptides for recognition by T cells in antigen-induced proliferation assays. Materials/Subjects and Methods: The complete sequence of Ag85B was analyzed for HLA-DR binding prediction to 51 HLA-DR alleles by using a virtual matrix-based prediction program (ProPred). Synthetic peptides covering the sequence of mature Ag85B were also analyzed for binding to HLA-DR alleles, and evaluated for recognition in antigen-induced proliferation assays with Ag85B-specific T-cell lines established from the peripheral blood mononuclear cells of 10 HLA-DR-heterogeneous tuberculosis patients. Results: The ProPred analysis of the full-length Ag85B (325 aa), signal peptide (40 aa) and the mature protein (285 aa) predicted their binding to 100, 76 and 98% of the 51 HLA-DR alleles, respectively. The analysis of 31 synthetic peptides for binding to HLA-DR alleles showed that 4 of them could bind >50% HLA-DR alleles, and were considered promiscuous. Testing of Ag85B-specific T-cell lines with synthetic peptides showed that all of the T-cell lines responded to one or more peptides of Ag85B, and 9 of the 10 cell lines responded to one or more of the four peptides considered promiscuous for binding to HLA-DR alleles. Conclusion: The ProPred program was useful in predicting the HLA-DR alleles binding regions of Ag85B and identifying the promiscuous peptides recognized by T cells.Copyright Copyright 2005 S. Karger AG, Basel
  • Identifier: Journal ISSN: 1011-7571
  • Publication Date: 2005
  • Physical Description: Electronic
  • Accrual Information: Quarterly
  • Shelfmark(s): 5531.260000
  • UIN: ETOCRN167720261

Searching Remote Databases, Please Wait