skip to main content
Show Results with:

Microbial surveillance during human pancreatic islet isolation

Bucher, P. et al.

Transplantation proceedings. VOL 36; NUMBER 4, ; 2004, 1147-1148 -- Elsevier Science B.V., Amsterdam. (pages 1147-1148) -- 2004

Online access

  • Title:
    Microbial surveillance during human pancreatic islet isolation
  • Author: Bucher, P.;
    Mathe, Z.;
    Bosco, D.;
    Oberholzer, J.;
    Toso, C.;
    Andres, A.;
    Buhler, L.;
    Morel, P.;
    Berney, T.
  • Found In: Transplantation proceedings. VOL 36; NUMBER 4, ; 2004, 1147-1148
  • Journal Title: Transplantation proceedings.
  • Subjects: Medicine; LCC: RD120; Dewey: 617.95
  • Publication Details: Elsevier Science B.V., Amsterdam.
  • Language: English
  • Abstract: ObjectivesThe study aim was to investigate the microbiological safety of islet isolation and transplantation.Materials and methodsBetween 1996 and 2002, prospective microbiological screening was performed on all pancreata procured for islet transplantation. Pancreas transport media and postpurification preparations were screened for microbiological contamination. Prior to isolation, pancreata were washed with either Hanks solution (group I, N = 170) or decontaminated with antiseptic and antimicrobial drugs (group II, N = 45).ResultsMicrobiological contamination of the pancreas preservation media was shown in 62%. Analysis of the contaminants showed 74% gram-positive, 21% gram-negative organisms, and 5% fungi. The donor condition or procurement center did not influence the contamination rate. Longer pancreas transport duration was significantly associated with bacterial contamination (P ConclusionsThe rate of microbial contamination during pancreas procurement and transport is high. Significant contaminants present when beginning islet isolation become undetectable by the conclusion of isolation. Diminishing the bio-burden by pancreas decontamination reduces the risk of contamination of the final islet preparation.
  • Identifier: Journal ISSN: 0041-1345
  • Publication Date: 2004
  • Physical Description: Electronic
  • Accrual Information: Quarterly
  • Shelfmark(s): 9025.100000
  • UIN: ETOCRN151051143

Searching Remote Databases, Please Wait