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Leishmania : methods and protocols / edited by Joachim Clos.

New York, NY : Humana Press, 2019.

Online access

  • Title:
    Leishmania : methods and protocols / edited by Joachim Clos.
  • Contributor: Joachim Clos editor.
  • Subjects: Leishmania -- Laboratory manuals; Electronic books;
    Dewey: 571.9/99
  • Rights: Terms governing use: Copyright.
    Access restrictions: NON_PRINT_LEGAL_DEPOSIT
  • Publication Details: New York, NY : Humana Press, 2019.
  • Language: English
  • Description: Contents: Preface…Table of Contents…Contributing Authors… 1. Host-Free Systems for Differentiation of Axenic LeishmaniaDan Zilberstein and Roni Nitzan Koren 2. Phylogenetic StudiesKatrin Kuhls and Isabel Mauricio 3. A Guide to Next Generation Sequence Analysis of Leishmania GenomesHideo Imamura and Jean-Claude Dujardin 4. Quantitative RNA Analysis using RNA SeqPeter J. Myler, Jacqueline A. McDonald, Pedro Alcolea, and Aakash Sur 5. Ribosome Profiling in TrypanosomatidsAmelie J. Kraus and Raúl O. Cosentino 6. Cosmid Library Construction and Functional CloningJoachim Clos and Dorotheo Zander-Dinse 7. Cos-Seq, A High Throughput Gain of Function Screen for Drug Resistance Studies in LeishmaniaJade-Eva Potvin, Philippe Leprohon, Elodie Gazanion, Mansi Sharma, Christopher Fernandez-Prada, and Marc Ouellette 8. Gene Replacement by Homologous RecombinationHenner Zirpel and Joachim Clos 9. LeishGEdit: A Method for Rapid Gene Knockout and Tagging using CRISPR-Cas9Tom Beneke and Eva Gluenz 10.
    Contents: DiCre-Based Inducible Disruption of Leishmania GenesSamuel M. Duncan, Elmarie Myburgh, Eliza Alves-Ferreira, and Jeremy C. Mottram 11. DiCre-Based Inducible Gene ExpressionJeziel D. Damasceno, Luiz R. O. Tosi, Renato E. R. S. Santos, and Jeremy C. Mottram 12. Generation of Bone Marrow-Derived Macrophages for In Vitro Infection ExperimentsEugenia Bifeld 13. Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR)Eugenia Bifeld 14. In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug ScreeningNuno Santarém, Joana Tavares, and Anabela Cordeiro-da-Silva 15. Quantification of Parasite Loads by Automated Microscopic Image AnalysisCarolina Borsoi Moraes and Laura Maria Alcântara 16. Quantification of Leishmania Parasites in Murine Models of Visceral InfectionJoana Tavares, Nuno Santarém, and Anabela Cordeiro-da-Silva 17.
    Contents: Syrian Hamster as an Advanced Experimental Model for Visceral LeishmaniasisMaría Dolores Jiménez-Antón, Montserrat Grau, Ana Isabel Olías-Molero, and José Mª Alunda 18. Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation versus Chronicity of DiseaseChristian Bogdan, Andrea Debus, Heidi Sebald, Bapli Rai, Johanna Schäfer, Stephanie Obermeyer, and Ulrike Schleicher 19. Establishment, Maintenance of Phlemotomus spp. In the Laboratory, and Infection with Leishmania spp.Ifhem Chelbi and Elyes Zhioua.
  • Identifier: ISBN 9781493992102 (electronic bk.); ISBN 1493992104 (electronic bk.); ISBN (print)
    10.1007/978-1-4939-9210-2 doi; BNB GBB979457; System number: 019370445
  • Notes: Includes bibliographical references and index.
  • Physical Description: 1 online resource (xiii, 370 pages) : illustrations (some color).
  • Series: Methods in molecular biology, volume 1971
  • Shelfmark(s): General Reference Collection DRT ELD.DS.411920
  • UIN: BLL01019370445

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