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Odontogenesis : methods and protocols / edited by Petros Papagerakis.

New York, NY : Humana Press : Springer, [2019].

Online access

  • Title:
    Odontogenesis : methods and protocols / edited by Petros Papagerakis.
  • Contributor: Petros Papagerakis editor.
  • Subjects: Teeth -- Growth -- Laboratory manuals; Odontogenesis; Tooth -- growth & develoment; Laboratory Manual; Electronic books;
    Dewey: 573.3/56
  • Rights: Terms governing use: Copyright.
    Access restrictions: NON_PRINT_LEGAL_DEPOSIT
  • Publication Details: New York, NY : Humana Press : Springer, [2019].
  • Language: English
  • Description: Contents: Microdissection and isolation of mouse dental epithelial cells of continuously growing mouse incisors / Establishment of an immortalized mouse bmp2 knockout dental papilla mesenchymal cell line / Establishment of stable cell lines from primary human dental pulp stem cells / Isolation of dental stem cell-enriched populations from continuously growing mouse incisors / Application of cell lineage tracing combined with immunofluorescence in the study of dentinogenesis / Tissue recombination and kidney capsule transplantation assays for the study of epithelial-mesenchymal interactions /
    Contents: Dental mesenchymal stem cells : dental pulp stem cells, periodontal ligament stem cells, apical papilla stem cells, and primary teeth stem cells-isolation, characterization, and expansion for tissue engineering / Phenotypic identification of dental pulp mesenchymal stem/stromal cells subpopulations with multiparametric flow cytometry / Dental pulp stem cells: isolation, characterization, expansion, and odontoblast differentiation for tissue engineering / In vitro analysis of intramolecular signaling events in PDLSCs using confocal and TIRF microscopy / Mouse model to study reparative dentinogenesis /
    Contents: Multiwalled carbon nanotubes for dental applications / Peptide-mediated biomimetic regrowth of human enamel in situ / Bioengineering tooth bud constructs using GelMA hydrogel / Whole-mount in situ hybridization of mouse embryos using DIG-labeled RNA probes / In situ hybridization on mouse paraffin sections using DIG-labeled RNA probes / Methods for in situ protein visualization in dental mineralized tissues / In situ hybridization in mineralized tissues : the added value of LNA probes for RNA detection /
    Contents: Immunofluorescence procedure for developing enamel tissues / Silver-albumin tissue staining protocol to visualize odontogenesis in whole embryos / Isolation of SIBLING proteins from bone and dentin matrices / Immunohistochemical co-localization of amelogenin and ameloblastin in developing enamel matrix / Expression and purification of recombinant mouse ameloblastin in E. coli / Protocols for Studying formation and mineralization of dental tissues in vivo : extraction protocol for isolating dentin matrix proteins from developing teeth / Purification of Developing enamel matrix proteins using preparative SDS-PAGE /
    Contents: Using imageJ (FIJI) to analyze and present x-ray CT images of enamel / Scanning electron microscopy (SEM) methods for dental enamel / Microcomputed tomography imaging in odontogenesis studies / Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) for the examination of dental hard tissues / Rodent dental fluorosis model: extraction of enamel organ from rat incisors / Three-dimensional assessment of crown size and eruption space for developing third molars : data collection techniques based on cone-beam computed tomography (CBCT) / Protocols to study dental caries in vitro : microbial caries models /
    Contents: In vitro caries models for the assessment of novel restorative materials / Protocols to study dental caries in vitro : pH cycling models / In vivo rodent models for studying dental caries and pulp disease / Protocol GenoDENT : implementation of a new NGS panel for molecular diagnosis of genetic disorders with orodental involvement /
    Contents: Protocols for genetic and epigenetic studies of rare diseases affecting dental tissues / Protocols, Methods, and tools for genome-wide association studies (GWAS) of dental traits / Measurement of early childhood oral health for research purposes: dental caries experience and developmental defects of the enamel in the primary dentition /
    Contents: Supragingival biofilm in early childhood caries : clinical and laboratory protocols and bioinformatics pipelines supporting metagenomics, metatranscriptomics, and metabolomics studies of the oral microbiome / Saliva and gingival crevicular fluid (GCF) collection for biomarker screening /
  • Identifier: ISBN 9781493990122 (electronic book); ISBN 1493990128 (electronic book); ISBN (hardcover); ISBN (hardcover); BNB GBB973017; System number: 019290942
  • Notes: Bibliography note: Includes bibliographical references and index.
  • Physical Description: 1 online resource (xviii, 565 pages) : illustrations (some color).
  • Series: Methods in molecular biology, 1922
  • Shelfmark(s): General Reference Collection DRT ELD.DS.393958
  • UIN: BLL01019290942

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