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Genetic Diversity in Commercial Rapeseed (Brassica napus L.) Varieties from Turkey as Revealed by RAPD

Notulae Scientia Biologicae, 2013, Vol.5(1), p.114 [Peer Reviewed Journal]

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  • Title:
    Genetic Diversity in Commercial Rapeseed (Brassica napus L.) Varieties from Turkey as Revealed by RAPD
  • Author: Oezbek, Oezlem ; Ucar Gidik, Betuel
  • Contributor: Oezbek, Oezlem (correspondence author)
  • Found In: Notulae Scientia Biologicae, 2013, Vol.5(1), p.114 [Peer Reviewed Journal]
  • Subjects: Data Processing ; Plant Breeding ; Genetic Diversity ; Genotypes ; Random Amplified Polymorphic DNA ; Oligonucleotides ; Crops ; Population Genetics ; Differentiation ; Principal Components Analysis ; Germplasm ; Gene Flow ; Primers ; Brassica Napus ; Plants and Algae
  • Language: English
  • Description: In cultivated commercial crop species, genetic diversity tends to decrease because of the extensive breeding processes. Therefore, germplasm of commercial crop species, such as Brassica napus L. should be evaluated and the genotypes, which have higher genetic diversity index, should be addressed as potential parental cross materials in breeding programs. In this study, the genetic diversity was analysed by using randomly amplified polymorphic DNA analysis (RAPD) technique in nine Turkish commercial rapeseed varieties. The RAPD primers (10-mer oligonucleotides) produced 51 scorable loci, 31 loci of which were polymorphic (60.78%) and 20 loci (39.22%) were monomorphic The RAPD bands were scored as binary matrix data and were analysed using POPGENE version 1.32. At locus level, the values of genetic diversity within population (Hs) and total (HT) were 0.15 and 0.19 respectively. The genetic differentiation (GST) and the gene flow (Nm) values between the populations were...
  • Identifier: ISSN: 2067-3205 ; E-ISSN: 2067-3264

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